NGS Data Analysis: Mastering the General Steps of Next-Generation Sequencing

This course provides a practical introduction to the foundational processes of Next-Generation Sequencing (NGS) data analysis. Designed for beginners in bioinformatics, it walks participants through every key stage – from understanding raw sequencing files to visualizing aligned reads.

This course covers the following topics:

• Fundamentals of Next-Generation Sequencing (NGS)

• Requirements and setup for starting NGS analysis

• Understanding the FASTQ file format

• Introduction to the SRA Toolkit for data retrieval

• Quality control of FASTQ files

• Performing read trimming and cleaning

• Conducting sequence alignment to a reference genome

• Understanding the SAM format and converting it to BAM

• Visualizing aligned reads using IGV (Integrative Genomics Viewer)

Learners will gain a clear, step-by-step understanding of the entire primary analysis pipeline, preparing them for deeper exploration into transcriptomic, genomic, or variant-level analyses.

Status:
This course is currently under production and will be available soon on AD Bioinformatics.